Determination of New Long-term Metabolites of Methylprednisolone In Human Urine by HPLC-ESI(-)/MS
نویسندگان
چکیده
Methylprednisolone synthetic corticosteroid (MP, Medrol, Canada 8 mg) was orally administered to healthy male volunteer. Urine samples were collected up to three days post-dose. Ten new metabolites have been identified in addition to the seven ones reported previously. LC-MS analysis have shown well-known 11-keto – 11-hydroxy interconversion and the formation of methylprednisolone 20-hydroxy, 6,7-dihydro and 20-hydroxy-6,7-dihydro metabolites. In addition formation a number of mono-hydroxylated metabolites of MP have been observed. We conclude that the new metabolites can be used for the detection of methylprednisolone abuse since the parent drug MP readily disappears in urine.
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